High resolution mapping of E-coli transcription elongation complex in situ reveals protein interactions with the non-transcribed strand

被引：23

作者：

Guerin, M ^{[1
]}

Leng, M ^{[1
]}

Rahmouni, AR ^{[1
]}

机构：

[1] CNRS, CTR BIOPHYS MOL, F-45071 ORLEANS 2, FRANCE

来源：

EMBO JOURNAL | 1996年 / 15卷 / 19期

关键词：

E-coli RNA polymerase; in situ chemical probing; transcription elongation complex;

D O I：

10.1002/j.1460-2075.1996.tb00923.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used chemical probes and UV light to perform a high resolution mapping of an Escherichia coli transcription elongation complex that was arrested in vivo by a protein readblock at a position distal to the promoter. The in situ probing data provide a precise picture of a constrained ternary complex in which the front edge of the polymerase is located at <6 bp from the catalytic center, Furthermore, our analyses reveal protein contacts with the non-transcribed strand within the arrested ternary complex, Thus, these results contribute substantially to the emerging view of a flexible transcription elongation complex in which the non-transcribed strand is an important regulatory element.

引用

页码：5397 / 5407

页数：11

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