Roles of disulfide bonds in bacterial alkaline phosphatase

被引:89
作者
Sone, M [1 ]
Kishigami, S [1 ]
Yoshihisa, T [1 ]
Ito, K [1 ]
机构
[1] KYOTO UNIV,INST VIRUS RES,DEPT CELL BIOL,KYOTO 60601,JAPAN
关键词
D O I
10.1074/jbc.272.10.6174
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alkaline phosphatase of Escherichia coli (a homodimeric protein found in the periplasmic space) contains two intramolecular disulfide bonds (Cys-168-Cys-178 and Cys-286-Cys-336) that are formed after export to the periplasmic space, The location-specific folding character of this enzyme allowed its wide usage as a reporter of protein localization in prokaryotic cells, To study the roles of disulfide bonds in alkaline phosphatase, we eliminated each of them by Cys to Ser mutations, Intracellular stability of alkaline phosphatase de creased in the absence of either one or both of the disulfide bonds, The mutant proteins were stabilized in a DegP protease-deficient strain, allowing accumulation at significant levels and subsequent characterization, A mutant protein that lacked the N-terminally located disulfide bond (Cys-168-Cys-178) was found to have Cys-286 and Cys-336 residues disulfide-bonded, to have a dimeric structure, and to have almost full enzymatic activity, Nevertheless, the mutant protein lost the trypsin-resistant conformation that is characteristically observed for the wild type enzyme, In contrast, mutants lacking Cys-286 and Cys-336 were monomeric and inactive, These results indicate that the Cys-286-Cys-336 disulfide bond is required and is sufficient for correctly positioning the active site region of this enzyme, but such an active conformation is still insufficient for the conformational stability of the enzyme, Thus, a fully active state of this enzyme can be formed without full protein stability, and the two disulfide bonds differentially contribute to these properties.
引用
收藏
页码:6174 / 6178
页数:5
相关论文
共 40 条
[1]  
AKIYAMA Y, 1989, J BIOL CHEM, V264, P437
[2]  
AKIYAMA Y, 1992, J BIOL CHEM, V267, P22440
[3]  
AKIYAMA Y, 1994, J BIOL CHEM, V269, P5218
[4]  
AKIYAMA Y, 1993, J BIOL CHEM, V268, P8146
[5]  
APPLEBURY ML, 1969, J BIOL CHEM, V244, P308
[6]   A PATHWAY FOR DISULFIDE BOND FORMATION INVIVO [J].
BARDWELL, JCA ;
LEE, JO ;
JANDER, G ;
MARTIN, N ;
BELIN, D ;
BECKWITH, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (03) :1038-1042
[7]   IDENTIFICATION OF A PROTEIN REQUIRED FOR DISULFIDE BOND FORMATION INVIVO [J].
BARDWELL, JCA ;
MCGOVERN, K ;
BECKWITH, J .
CELL, 1991, 67 (03) :581-589
[8]  
Bollag D.M., 1991, PROTEIN METHODS, P143
[9]  
Boyd D, 1987, PHOSPHATE METABOLISM, P89
[10]   AMINO-ACID-SEQUENCE OF ESCHERICHIA-COLI ALKALINE-PHOSPHATASE [J].
BRADSHAW, RA ;
CANCEDDA, F ;
ERICSSON, LH ;
NEUMANN, PA ;
PICCOLI, SP ;
SCHLESINGER, MJ ;
SHRIEFER, K ;
WALSH, KA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (06) :3473-3477