A highly regioselective synthesis of mannobiose and mannotriose by reverse hydrolysis using specific 1,2-alpha-mannosidase from Aspergillus phoenicis

被引：21

作者：

Suwasono, S ^{[1
]}

Rastall, RA ^{[1
]}

机构：

[1] UNIV READING,DEPT FOOD SCI & TECHNOL,BIOTECHNOL & BIOCHEM ENGN GRP,READING RG6 6AP,BERKS,ENGLAND

来源：

BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 07期

关键词：

D O I：

10.1007/BF00127901

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A specific 1,2-alpha-mannosidase was isolated from A. phoenicis and used in the equilibrium-controlled synthesis of alpha 1-->2-linked mannobiose and mannotriose. Yields of 22.33% disaccharide, 8.21% trisaccharide and 2.74% tetrasaccharide were obtained. Regioselectivity was absolute for the 1-->2 linkage.

引用

页码：851 / 856

页数：6

共 10 条

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