Diffusion in Pseudomonas aeruginosa biofilms:: a pulsed field gradient NMR study

被引:60
作者
Vogt, M
Flemming, HC
Veeman, WS
机构
[1] Univ Duisburg Gesamthsch, Inst Theoret & Phys Chem, D-47057 Duisburg, Germany
[2] Univ Duisburg Gesamthsch, Fac Aquat Microbiol, D-47057 Duisburg, Germany
关键词
PFG-NMR; diffusion; water; biofilm; Pseudomonas aeruginosa;
D O I
10.1016/S0168-1656(99)00213-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A Pseudomonas aeruginosa biofilm is studied with pulsed field gradient echo nuclear magnetic resonance. Although not all spectral components are assigned yet, the experimental results show that a biofilm consists of components with very different diffusion coefficients. The various biofilm components that give motionally narrowed H-1 NMR signals, can be grouped into five classes with diffusion coefficients, ranging from 2 x 10(-9) to 1 x 10(-13) m(2) s(-1). Investigation of the diffusion behavior of water in the biofilm shows three fractions with different diffusion coefficients. Besides the highly mobile bulk water at least two other fractions with much lower diffusion coefficients are detected. It is shown that one of the fractions with the low diffusion coefficient probably arises from intracellular water. Also for another component of the biofilm, glycerol, three fractions with diffusion coefficients that differ more than a factor ten are detected. Also a group of signals exists which result from practically immobile components. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:137 / 146
页数:10
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