Insertion of an amino acid in the DNA-binding domain of the glucocorticoid receptor as a result of alternative splicing

被引：109

作者：

Rivers, C

Levy, A

Hancock, J

Lightman, S

Norman, M

机构：

[1] Univ Bristol, Div Med, Bristol BS8 1TH, Avon, England

[2] Univ Bristol, Dept Pathol & Microbiol, Bristol BS8 1TH, Avon, England

来源：

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM | 1999年 / 84卷 / 11期

关键词：

D O I：

10.1210/jc.84.11.4283

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

When the human glucocorticoid receptor (GR) was first sequenced, a predominant form (GR alpha) and a minor variant (GR beta) were identified (1). In the present communication, we describe a new variant of the glucocorticoid receptor (GR gamma) in which, as a result of alternative splicing, three bases are retained from the intron separating exons 3 and 4. These three bases code for an additional amino acid (arginine) in the DNA binding domain of the receptor. Insertion of arginine at this site has previously been shown to decrease transcriptional activation by the GR to 48% that of GR alpha (2). Analysis of cDNA from different tissues shows that the novel form is widely expressed at a relatively high level (between 3.8 and 8.7% of total GR).

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页码：4283 / 4286

页数：4

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