Characterization of a human pancreatic secretory trypsin inhibitor mutant binding to Legionella pneumophila as determined by a quartz crystal microbalance

被引:14
作者
Decker, J
Weinberger, K
Prohaska, E
Hauck, S
Kösslinger, C
Wolf, H
Hengerer, A
机构
[1] Univ Regensburg, Inst Med Microbiol & Hyg, D-93053 Regensburg, Germany
[2] Fraunhofer Inst Solid State Technol, D-80686 Munich, Germany
关键词
affinity constant; human pancreatic secretory trypsin inhibitor; immunosensor; phage display; quartz crystal microbalance;
D O I
10.1016/S0022-1759(99)00187-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We describe the isolation from a large phagemid library of a human pancreatic secretory trypsin inhibitor (hPSTI) mutant that binds to Legionella pneumophila. To gain further insight into the binding kinetics of the isolated hPSTI mutant, an immunosensing system based on a quartz crystal microbalance (QCM) was used. In contrast to ELISA procedures, k(on) and k(off) rates could be derived from the QCM sensograms. Thus, it is possible to characterize specific intermolecular interactions between proteins and phages isolated from large phage display libraries by QCM. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:159 / 165
页数:7
相关论文
共 12 条
  • [1] PHAGE LIBRARIES FOR GENERATION OF CLINICALLY USEFUL ANTIBODIES
    CHESTER, KA
    BEGENT, RHJ
    ROBSON, L
    KEEP, P
    PEDLEY, RB
    BODEN, JA
    BOXER, G
    GREEN, A
    WINTER, G
    COCHET, O
    HAWKINS, RE
    [J]. LANCET, 1994, 343 (8895) : 455 - 456
  • [2] COLLINS J, 1990, BIOL CHEM H-S, V371, P29
  • [3] EHRET W, 1985, ZBL BAKT-INT J MED M, V260, P184
  • [4] EHRET W, 1985, ZBL BAKT-INT J MED M, V259, P433
  • [5] SELECTION OF PHAGE ANTIBODIES BY BINDING-AFFINITY - MIMICKING AFFINITY MATURATION
    HAWKINS, RE
    RUSSELL, SJ
    WINTER, G
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1992, 226 (03) : 889 - 896
  • [6] KOSSLINGER C, 1995, SENSOR ACTUAT B-CHEM, V24, P107, DOI 10.1016/0925-4005(95)85023-6
  • [7] PHAGE ANTIBODIES - FILAMENTOUS PHAGE DISPLAYING ANTIBODY VARIABLE DOMAINS
    MCCAFFERTY, J
    GRIFFITHS, AD
    WINTER, G
    CHISWELL, DJ
    [J]. NATURE, 1990, 348 (6301) : 552 - 554
  • [8] A HUMAN PANCREATIC SECRETORY TRYPSIN-INHIBITOR PRESENTING A HYPERVARIABLE HIGHLY CONSTRAINED EPITOPE VIA MONOVALENT PHAGEMID DISPLAY
    ROTTGEN, P
    COLLINS, J
    [J]. GENE, 1995, 164 (02) : 243 - 250
  • [9] VERWENDUNG VON SCHWINGQUARZEN ZUR WAGUNG DUNNER SCHICHTEN UND ZUR MIKROWAGUNG
    SAUERBREY, G
    [J]. ZEITSCHRIFT FUR PHYSIK, 1959, 155 (02): : 206 - 222
  • [10] Schier Robert, 1996, Human Antibodies and Hybridomas, V7, P97