Binding of Streptococcus gordonii to extracellular matrix proteins

被引：28

作者：

Giomarelli, Barbara

Visai, Livia

Hijazi, Karolin

Rindi, Simonetta

Ponzio, Michela

Iannelli, Francesco

Speziale, Pietro

Pozzi, Gianni

机构：

[1] Univ Siena, LAMMB, Policlin Le Scotte V Lotto, Dept Mol Biol, I-53100 Siena, Italy

[2] Univ Pavia, Dept Biochem, I-27100 Pavia, Italy

[3] Univ Pavia, Sect Epidemiol & Med Stat, Dept Hlth Sci, I-27100 Pavia, Italy

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 265卷 / 02期

关键词：

Streptococcus gordonii; oral streptococci; extracellular matrix proteins (EMPs); microbial surface components recognizing adhesive matrix molecules (MSCRAMMs); microbial adhesins;

D O I：

10.1111/j.1574-6968.2006.00479.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Knock-out mutants of Streptococcus gordonii Challis were constructed and assayed for binding to extracellular matrix proteins (EMPs) by enzyme-linked immunosorbent assay (ELISA). It was shown that (i) the mutant lacking the cell wall polysaccharide receptor could no longer bind type I and type II collagen, (ii) the mutant lacking the fibronectin-binding proteins CshA and FbpA was also strongly impaired in collagen binding and (iii) the mutant lacking the methionine sulfoxide reductase MsrA was significantly impaired in fibronectin binding. Our results indicate that binding to EMPs by S. gordonii is a multifactorial process controlled by genes located at three different chromosomal sites.

引用

页码：172 / 177

页数：6