Long-range heterogeneity at the 3′ ends of human mRNAs

被引：62

作者：

Iseli, C ^{[1
]}

Stevenson, BJ ^{[1
]}

de Souza, SJ ^{[1
]}

Samaia, HB ^{[1
]}

Camargo, AA ^{[1
]}

Buetow, KH ^{[1
]}

Strausberg, RL ^{[1
]}

Simpson, AJG ^{[1
]}

Bucher, P ^{[1
]}

Jongeneel, CV ^{[1
]}

机构：

[1] NCI, Bethesda, MD 20892 USA

来源：

GENOME RESEARCH | 2002年 / 12卷 / 07期

关键词：

D O I：

10.1101/gr.62002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The publication of a draft of the human genome and of large collections of transcribed sequences has made it possible to study the complex relationship between the transcriptome and the genome. In the work presented here, we have focused on mapping mRNA 3' ends onto the genome by use of the raw data generated by the expressed sequence tag (EST) sequencing projects. We Find that at least half of the human genes encode multiple transcripts whose polyadenylation is driven by multiple signals. The corresponding transcript 3' ends are spread over distances in the kilobase range. This finding has profound implications for Our understanding of gene expression regulation and of the diversity of human transcripts, for the design of cDNA microarray probes, and for the interpretation of gene expression profiling experiments.

引用

页码：1068 / 1074

页数：7

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