Poly(ethylene glycol)-lipase complex that is catalytically active for alcoholysis reactions in ionic liquids

被引：76

作者：

Maruyama, T ^{[1
]}

Nagasawa, S ^{[1
]}

Goto, M ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Engn, Dept Appl Chem, Fukuoka 8128581, Japan

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 16期

关键词：

alcoholysis; ionic liquid; lipase; polyethylene glycol;

D O I：

10.1023/A:1019848400436

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lipase-catalyzed alcoholysis was investigated in three different ionic liquids. Lyophilized native lipase had a low activity in all the ionic liquids but a poly(ethylene glycol) (PEG)-lipase complex (with a molar ratio of the polymer/enzyme of 10:1) had an increased activity of over 14-fold. Of several lipases tested, PEG-lipase PS (from Pseudomonas cepacia) exhibited the highest activity (1.07 mmol/(h g(-1) protein)) in 1-octyl-3-methylimidazolium hexafluorophosphate.

引用

页码：1341 / 1345

页数：5

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