Generation of a large complex antibody library from multiple donors

被引：45

作者：

Little, M ^{[1
]}

Welschof, M

Braunagel, M

Hermes, I

Christ, C

Keller, A

Rohrbach, P

Kürschner, T

Schmidt, S

Kleist, C

Terness, P

机构：

[1] German Canc Res Ctr, Recombinant Antibody Univ D0500, Diagnost & Expt therpay Programme, INF 280, D-69120 Heidelberg, Germany

[2] Univ Heidelberg, Dept Transplantat Immunol, Inst Immunol, D-69120 Heidelberg, Germany

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1999年 / 231卷 / 1-2期

关键词：

antibody library; phage display; human IgM and IgG repertoire; single chain antibody;

D O I：

10.1016/S0022-1759(99)00164-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have generated a large complex library of single chain antibodies based on four individual libraries from each of 50 donors. DNA coding for the heavy and light chain variable domains of the IgM and IgG repertoires was amplified by PCR using two different sets of primers. Each individual library was composed of approximately 1-5 x 10(7) independent clones giving a final combined library of 4 x 10(9) members. Screening this library by phase display of single chain antibodies with small haptens, peptides and proteins yielded specific antibodies for each class of antigen. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：3 / 9

页数：7

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