Molecular beacons: Trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria

被引：23

作者：

Schofield, P ^{[1
]}

Pell, AN ^{[1
]}

Krause, DO ^{[1
]}

机构：

[1] CORNELL UNIV,DEPT ANIM SCI,ITHACA,NY 14853

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1997年 / 63卷 / 03期

关键词：

D O I：

10.1128/AEM.63.3.1143-1147.1997

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Molecular beacons are fluorescent probes developed for solution rather than membrane hybridization. We have investigated the utility of these probes to study rumen microbial ecology. Two cellulolytic species, Ruminococcus albus and Fibrobacter succinogenes, were tested, Membrane and solution hybridizations gave similar results in competition experiments with cocultures of R. albus 8 and F. succinogenes S85.

引用

页码：1143 / 1147

页数：5

共 15 条

[1] FLUORESCENT-OLIGONUCLEOTIDE PROBING OF WHOLE CELLS FOR DETERMINATIVE, PHYLOGENETIC, AND ENVIRONMENTAL-STUDIES IN MICROBIOLOGY [J].