Molecular beacons: Trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria

被引:23
作者
Schofield, P [1 ]
Pell, AN [1 ]
Krause, DO [1 ]
机构
[1] CORNELL UNIV,DEPT ANIM SCI,ITHACA,NY 14853
关键词
D O I
10.1128/AEM.63.3.1143-1147.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Molecular beacons are fluorescent probes developed for solution rather than membrane hybridization. We have investigated the utility of these probes to study rumen microbial ecology. Two cellulolytic species, Ruminococcus albus and Fibrobacter succinogenes, were tested, Membrane and solution hybridizations gave similar results in competition experiments with cocultures of R. albus 8 and F. succinogenes S85.
引用
收藏
页码:1143 / 1147
页数:5
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