Concurrent versus individual binding of HuR and AUF1 to common labile target mRNAs

被引：392

作者：

Lal, A ^{[1
]}

Mazan-Mamczarz, K ^{[1
]}

Kawai, T ^{[1
]}

Yang, XL ^{[1
]}

Martindale, JL ^{[1
]}

Gorospe, M ^{[1
]}

机构：

[1] NIA, LCMB, IRP, NIH, Baltimore, MD 21224 USA

来源：

EMBO JOURNAL | 2004年 / 23卷 / 15期

关键词：

exosome; mRNA stability; polysome; RNA-binding protein; RNA motif;

D O I：

10.1038/sj.emboj.7600305

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA-binding proteins HuR and AUF1 bind to many common AU-rich target mRNAs and exert opposing influence on target mRNA stability, but the functional interactions between HuR and AUF1 have not been systematically studied. Here, using common target RNAs encoding p21 and cyclin D1, we provide evidence that HuR and AUF1 can bind target transcripts on both distinct, nonoverlapping sites, and on common sites in a competitive fashion. In the nucleus, both proteins were found together within stable ribonucleoprotein complexes; in the cytoplasm, HuR and AUF1 were found to bind to target mRNAs individually, HuR colocalizing with the translational apparatus and AUF1 with the exosome. Our results indicate that the composition and fate (stability, translation) of HuR- and/or AUF1-containing ribonucleoprotein complexes depend on the target mRNA of interest, RNA-binding protein abundance, stress condition, and subcellular compartment.

引用

页码：3092 / 3102

页数：11

共 47 条

[1] Embryonic lethal abnormal visual RNA-binding proteins involved in growth, differentiation, and posttranscriptional gene expression [J].