Bovine serum albumin, α-lactoalbumin and β-lactoglobulin partitioning in polyethylene glycol/maltodextrin aqueous-two-phase systems

被引:18
作者
da Silva, LHM [1 ]
Meirelles, AJA [1 ]
机构
[1] Univ Estadual Campinas, Food Engn Dept FEA, LASEFI Phys Separat Lab, BR-13083970 Campinas, SP, Brazil
关键词
partitioning; aqueous two-phase systems; bovine serum albumin; alpha-lactoalbumin; beta-lactoglobulin;
D O I
10.1016/S0144-8617(99)00177-0
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The polysaccharide maltodextrin (MD) can provide a low cost alternative to substitute the fractioned dextran for the use with polyethylene glycol (PEG) in aqueous two-phase, two-polymers systems. Ln this work we have studied the partitioning of Bovine Serum Albumin (BSA), alpha-Lactoalbumin (alpha-La) and beta-Lactoglobulin (beta-Lg) in PEG/MD aqueous two-phase systems. The experiments were performed at local atmospheric pressure (727 mmHg) and 25 degrees C. The influence of the polymers molecular weights and tie-line length were investigated. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:279 / 282
页数:4
相关论文
共 17 条
  • [1] Albertsson PA, 1986, PARTITION CELL PARTI, V3
  • [2] Atkinson L., 1994, Food and Bioproducts Processing, V72, P106
  • [3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [4] CHEN JP, 1992, J FERMENT BIOENG, V73, P140, DOI 10.1016/0922-338X(92)90579-J
  • [5] CHISTIAN TJ, 1998, CARBOHYD POLYM, V35, P7
  • [6] Coimbra JD, 1995, BIOSEPARATION, V5, P259
  • [7] Dispersed phase hold-up in a perforated rotating disc contactor (PRDC) using aqueous two-phase systems
    Coimbra, JSR
    Mojola, F
    Meirelles, AJA
    [J]. JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 1998, 31 (02) : 277 - 280
  • [8] FISHER D, 1981, BIOCHEM J, V196, P1
  • [9] FORCINITI D, 1991, BIOSEPARATION, V2, P115
  • [10] Johansson G., 1985, PARTITIONING AQUEOUS, P161