Targeted development of microsatellite markers from the defined region of bovine Chromosome 6q21-31

被引：28

作者：

Weikard, R ^{[1
]}

Goldammer, T ^{[1
]}

Kuhn, C ^{[1
]}

Barendse, W ^{[1
]}

Schwerin, M ^{[1
]}

机构：

[1] UNIV QUEENSLAND,CSIRO TROP AGR,GEHRMAN LABS,ST LUCIA,QLD 4072,AUSTRALIA

来源：

MAMMALIAN GENOME | 1997年 / 8卷 / 11期

关键词：

D O I：

10.1007/s003359900588

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A methodical strategy for the isolation of microsatellite markers specific for targeted regions of bovine chromosomes is presented. The procedure involves directed microdissection of one defined subchromosomal area, its DOP-PCR-amplification and cloning, With this approach, a library specific to the BTA 6q21-31 chromosomal region was constructed. Eleven unique microsatellite-containing sequences were isolated, converted into sequence-tagged microsatellite sites, and characterized concerning their species-specific origin. Seven primer pairs generated bovine-specific PCR products and provided a set of microsatellite markers that generally revealed high informativity in the HF breed. Linkage analysis assigned six of them to their predefined subchromosomal origin on BTA 6 corresponding to the specific rehybridization signal of the DOP-PCR product generated from the microdissected chromosome area 6q21-31. The results underline the usefulness of the BTA 6q21-31 library for targeted isolation of unique sequences that are specific for the dissected chromosomal region as demonstrated here by the isolation of microsatellite markers.

引用

页码：836 / 840

页数：5

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