In situ hybridisation mapping of genomic clones for five human respiratory chain complex I genes

被引：6

作者：

Dunbar, DR

Shibasaki, Y

Dobbie, L

Andersson, B

Brookes, AJ

机构：

[1] BIOMED CTR,DEPT MED GENET,S-75123 UPPSALA,SWEDEN

[2] WESTERN GEN HOSP,MRC,HUMAN GENET UNIT,EDINBURGH EH4 2XU,MIDLOTHIAN,SCOTLAND

来源：

CYTOGENETICS AND CELL GENETICS | 1997年 / 78卷 / 01期

关键词：

D O I：

10.1159/000134618

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Using human and bovine short cDNA sequences as probes we screened human cosmid and P1 libraries for components of the complex I multi-subunit enzyme of oxidative phosphorylation. We isolated genomic recombinants encoding cI-B8 (gene NDUFA2), cI-B14 (gene NDUFA6), cI-B14.5a (gene NDUFA7), cI-ASHI (gene NDUFB8) and cI-23kD (gene NDUFS8). Genomic versions of these genes have not been previously cloned in the human although they are represented as anonymous entries in public cDNA databases. By using the derived genomic clones for in situ hybridisation studies we determined the following chromosome locations: NDUFA2, 5q31; NDUFA6, 21q22; NDUFA7, 20p13; NDUFB8, 12q21; NDUFS8, 3q28.

引用

页码：21 / 24

页数：4

共 30 条

[1] CHROMOSOMAL LOCALIZATION OF THE HUMAN GENE ENCODING THE 51-KDA SUBUNIT OF MITOCHONDRIAL COMPLEX-I (NDUFV1) TO 11Q13 [J].