Collaborative study of the NCCLS and flow cytometry methods for antifungal susceptibility testing of Candida albicans

被引:32
作者
Chaturvedi, V
Ramani, R
Pfaller, MA
机构
[1] New York State Dept Hlth, Wadsworth Ctr, Mycol Lab, Albany, NY 12208 USA
[2] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY USA
[3] Univ Iowa, Coll Med, Dept Pathol, Iowa City, IA 52242 USA
[4] Univ Iowa, Coll Med, Dept Epidemiol, Iowa City, IA 52242 USA
[5] Coll Publ Hlth, Iowa City, IA USA
关键词
D O I
10.1128/JCM.42.5.2249-2251.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
One hundred clinical isolates of Candida albicans were tested for amphotericin B and fluconazole susceptibilities by the National Committee for Clinical Laboratory Standards (NCCLS) broth microdilution test at center 1 (C1). The same isolates were tested blinded at center 2 (C2) by NCCLS and flow cytometry (FC) methods. The agreement between NCCLS and FC methods ranged from 96 to 99%.
引用
收藏
页码:2249 / 2251
页数:3
相关论文
共 17 条
[1]   Comparison of visual and spectrophotometric methods of broth microdilution MIC end point determination and evaluation of a sterol quantitation method for in vitro susceptibility testing of fluconazole and itraconazole against trailing and nontrailing Candida isolates [J].
Arthington-Skaggs, BA ;
Lee-Yang, W ;
Ciblak, MA ;
Frade, JP ;
Brandt, ME ;
Hajjeh, RA ;
Harrison, LH ;
Sofair, AN ;
Warnock, DW .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2002, 46 (08) :2477-2481
[2]   Fluconazole disk diffusion procedure for determining susceptibility of Candida species [J].
Barry, AL ;
Brown, SD .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (09) :2154-2157
[3]   Comparison of a photometric method with standardized methods of antifungal susceptibility testing of yeasts [J].
Clancy, CJ ;
Nguyen, MH .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (11) :2878-2882
[4]   Amphotericin B susceptibility testing of Candida lusitaniae isolates by flow cytofluorometry:: comparison with the Etest and the NCCLS broth macrodilution method [J].
Favel, A ;
Peyron, F ;
De Méo, M ;
Michel-Nguyen, A ;
Carrière, J ;
Chastin, C ;
Regli, P .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1999, 43 (02) :227-232
[5]   RAPID-DETERMINATION OF ANTIFUNGAL ACTIVITY BY FLOW-CYTOMETRY [J].
GREEN, L ;
PETERSEN, B ;
STEIMEL, L ;
HAEBER, P ;
CURRENT, W .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (04) :1088-1091
[6]   Rapid susceptibility testing of Candida albicans by flow cytometry [J].
Kirk, SM ;
Callister, SM ;
Lim, LCL ;
Schell, RF .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (02) :358-363
[7]  
National Committee for Clinical Laboratory Standards, 2002, M27A2 NAT COMM CLIN
[8]   Evaluation of a flow cytofluorometric method for rapid determination of amphotericin B susceptibility of yeast isolates [J].
Peyron, F ;
Favel, A ;
GuiraudDauriac, H ;
ElMzibri, M ;
Chastin, C ;
Dumenil, G ;
Regli, P .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (07) :1537-1540
[9]   Clinical evaluation of the ASTY colorimetric microdilution panel for antifungal susceptibility testing [J].
Pfaller, MA ;
Arikan, S ;
Lozano-Chiu, M ;
Chen, YS ;
Coffman, S ;
Messer, SA ;
Rennie, R ;
Sand, C ;
Heffner, T ;
Rex, JH ;
Wang, J ;
Yamane, N .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (09) :2609-2612
[10]   Multisite reproducibility of the Etest MIC method for antifungal susceptibility testing of yeast isolates [J].
Pfaller, MA ;
Messer, SA ;
Bolmstrom, A ;
Odds, FC ;
Rex, JH .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (07) :1691-1693