Functional genomic analysis of amniotic fluid cell-free mRNA suggests that oxidative stress is significant in Down syndrome fetuses

被引:95
作者
Slonim, Donna K. [2 ,3 ]
Koide, Keiko [1 ]
Johnson, Kirby L. [1 ]
Tantravahi, Umadevi [4 ]
Cowan, Janet M. [1 ]
Jarrah, Zina [1 ]
Bianchi, Diana W. [1 ]
机构
[1] Floating Hosp Children, Tufts Med Ctr, Dept Pediat, Div Genet, Boston, MA 02111 USA
[2] Tufts Univ, Sch Med, Dept Pathol, Boston, MA 02111 USA
[3] Tufts Univ, Dept Comp Sci, Medford, MA 02155 USA
[4] Women & Infants Hosp Rhode Isl, Dept Pathol, Providence, RI 02905 USA
关键词
antenatal therapy; Connectivity Map; gene expression; prenatal diagnosis; trisomy; 21; GENE-EXPRESSION ANALYSIS; TRANSCRIPTIONAL CHANGES; PROTEIN FAMILIES; CONNECTIVITY MAP; OVER-EXPRESSION; TS1CJE MOUSE; MODEL; BRAIN; CHROMOSOME-21; APOPTOSIS;
D O I
10.1073/pnas.0903909106
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
To characterize the differences between second trimester Down syndrome (DS) and euploid fetuses, we used Affymetrix microarrays to compare gene expression in uncultured amniotic fluid supernatant samples. Functional pathway analysis highlighted the importance of oxidative stress, ion transport, and G protein signaling in the DS fetuses. Further evidence supporting these results was derived by correlating the observed gene expression patterns to those of small molecule drugs via the Connectivity Map. Our results suggest that there are secondary adverse consequences of DS evident in the second trimester, leading to testable hypotheses about possible antenatal therapy for DS.
引用
收藏
页码:9425 / 9429
页数:5
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