Genetic dissection of complex traits with chromosome substitution strains of mice

被引：259

作者：

Singer, JB

Hill, AE

Burrage, L

Olszens, KR

Song, JH

Justice, M

O'Brien, WE

Conti, DV

Witte, JS

Lander, ES ^{[1
]}

Nadeau, JH

机构：

[1] Broad Inst, Cambridge, MA 02142 USA

[2] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[3] Case Western Reserve Univ, Sch Med, Dept Genet, Cleveland, OH 44106 USA

[4] Case Western Reserve Univ, Ctr Computat Genom & Syst Biol, Cleveland, OH 44106 USA

[5] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA

[6] Case Western Reserve Univ, Dept Epidemiol & Biostat, Cleveland, OH 44106 USA

[7] MIT, Dept Biol, Cambridge, MA 02139 USA

[8] Univ Hosp Cleveland, Ctr Human Genet, Cleveland, OH 44106 USA

来源：

SCIENCE | 2004年 / 304卷 / 5669期

关键词：

D O I：

10.1126/science.1093139

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Chromosome substitution strains (CSSs) have been proposed as a simple and powerful way to identify quantitative trait loci (QTLs) affecting developmental, physiological, and behavioral processes. Here, we report the construction of a complete CSS panel for a vertebrate species. The CSS panel consists of 22 mouse strains, each of which carries a single chromosome substituted from a donor strain (A/J) onto a common host background (C57BL/6J). A survey of 53 traits revealed evidence for 150 QTLs affecting serum levels of sterols and amino acids, diet-induced obesity, and anxiety. These results demonstrate that CSSs greatly facilitate the detection and identification of genes that control the wide diversity of naturally occurring phenotypic variation in the A/J and C57BL/6J inbred strains.

引用

页码：445 / 448

页数：4

共 22 条

[1]

[Anonymous], 2000, What's Wrong with my Mouse?

[2]

ASHBURNER M, 1997, GENETICS BIOL DROSOP

[3] Chromosome substitution strains: some quantitative considerations for genome scans and fine mapping [J].