Progress toward an explicit mechanistic model for the light-driven pump, bacteriorhodopsin

被引:49
作者
Lanyi, JK [1 ]
机构
[1] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92697 USA
关键词
bacteriorhodopsin; membrane protein; proton transport; bioenergetics;
D O I
10.1016/S0014-5793(99)01685-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recent crystallographic information about the structure of bacteriorhodopsin and some of its photointermediates, together with a large amount of spectroscopic and mutational data, suggest a mechanistic model for how this protein couples light energy to the translocation of protons across the membrane. Now nearing completion, this detailed molecular model will describe the nature of the steric and electrostatic conflicts at the photoisomerized retinal, as well as the means by which it induces proton transfers in the two half-channels leading to the two membrane surfaces, thereby causing unidirectional, uphill transport. (C) 1999 Federation of European Biochemical Societies.
引用
收藏
页码:103 / 107
页数:5
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