Stable Lariat Formation Based on a G-Quadruplex Scaffold

被引:33
作者
Xu, Yan [1 ]
Sato, Hiroyuki [1 ]
Sannohe, Yuta [1 ]
Shinohara, Ken-ichi [1 ]
Sugiyama, Hiroshi [1 ]
机构
[1] Kyoto Univ, Grad Sch Sci, Dept Chem, Sakyo Ku, Kyoto 6068502, Japan
关键词
D O I
10.1021/ja806535j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In the telomere region, T-loop structure is thought to participate in protecting chromosome ends. Although the tetraplex structure, called G-quadruplex, is suggested to form in the same region, the relationship between these two structures are less discussed. Here we designed the long lariat formation using G-quadruplex structure. The structural property was characterized by means of CD spectroscopy, FRET experiment, and native PAGE analysis. Because this lariat formation contains both loop structure and tetraplex structure, it can be a key structure linking T-loop structure to G-quadrurplex structure.
引用
收藏
页码:16470 / +
页数:3
相关论文
共 13 条
[1]   Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution [J].
Ambrus, Attila ;
Chen, Ding ;
Dai, Jixun ;
Bialis, Tiffanie ;
Jones, Roger A. ;
Yang, Danzhou .
NUCLEIC ACIDS RESEARCH, 2006, 34 (09) :2723-2735
[2]   Mammalian telomeres end in a large duplex loop [J].
Griffith, JD ;
Comeau, L ;
Rosenfield, S ;
Stansel, RM ;
Bianchi, A ;
Moss, H ;
de Lange, T .
CELL, 1999, 97 (04) :503-514
[3]   TELOMERES SHORTEN DURING AGING OF HUMAN FIBROBLASTS [J].
HARLEY, CB ;
FUTCHER, AB ;
GREIDER, CW .
NATURE, 1990, 345 (6274) :458-460
[4]   SPECIFIC ASSOCIATION OF HUMAN TELOMERASE ACTIVITY WITH IMMORTAL CELLS AND CANCER [J].
KIM, NW ;
PIATYSZEK, MA ;
PROWSE, KR ;
HARLEY, CB ;
WEST, MD ;
HO, PLC ;
COVIELLO, GM ;
WRIGHT, WE ;
WEINRICH, SL ;
SHAY, JW .
SCIENCE, 1994, 266 (5193) :2011-2015
[5]   Structure of the human telomere in K+ solution:: An intramolecular (3+1) G-quadruplex scaffold [J].
Luu, Kim Ngoc ;
Phan, Anh Tuan ;
Kuryavyi, Vitaly ;
Lacroix, Laurent ;
Patel, Dinshaw J. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (30) :9963-9970
[6]   Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening [J].
Makarov, VL ;
Hirose, Y ;
Langmore, JP .
CELL, 1997, 88 (05) :657-666
[7]   The terminal DNA structure of mammalian chromosomes [J].
McElligott, R ;
Wellinger, RJ .
EMBO JOURNAL, 1997, 16 (12) :3705-3714
[8]   A HIGHLY CONSERVED REPETITIVE DNA-SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN-CHROMOSOMES [J].
MOYZIS, RK ;
BUCKINGHAM, JM ;
CRAM, LS ;
DANI, M ;
DEAVEN, LL ;
JONES, MD ;
MEYNE, J ;
RATLIFF, RL ;
WU, JR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :6622-6626
[9]   Telomestatin, a novel telomerase inhibitor from Streptomyces anulatus [J].
Shin-ya, K ;
Wierzba, K ;
Matsuo, K ;
Ohtani, T ;
Yamada, Y ;
Furihata, K ;
Hayakawa, Y ;
Seto, H .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (06) :1262-1263
[10]   Replication and protection of telomeres [J].
Verdun, Ramiro E. ;
Karlseder, Jan .
NATURE, 2007, 447 (7147) :924-931