The CENP-S complex is essential for the stable assembly of outer kinetochore structure

被引：113

作者：

Amano, Miho ^{[1
]}

Suzuki, Aussie ^{[1
]}

Hori, Tetsuya ^{[1
]}

Backer, Chelsea ^{[2
,3
]}

Okawa, Katsuya ^{[4
]}

Cheeseman, Iain M. ^{[2
,3
]}

Fukagawa, Tatsuo ^{[1
]}

机构：

[1] Grad Univ Adv Studies, Dept Mol Genet, Natl Inst Genet, Mishima, Shizuoka 4118540, Japan

[2] MIT, Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[3] MIT, Dept Biol, Cambridge, MA 02142 USA

[4] Kyoto Univ, Grad Sch Med, Sakyo Ku, Kyoto 6068501, Japan

来源：

JOURNAL OF CELL BIOLOGY | 2009年 / 186卷 / 02期

关键词：

CONSTITUTIVE CENTROMERE COMPONENT; CHROMOSOME SEGREGATION; VERTEBRATE CELLS; CHECKPOINT; PATHWAYS; HEC1;

D O I：

10.1083/jcb.200903100

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The constitutive centromere-associated network (CCAN) proteins are central to kinetochore assembly. To define the molecular architecture of this critical kinetochore network, we sought to determine the full complement of CCAN components and to define their relationships. This work identified a centromere protein S (CENP-S)-containing subcomplex that includes the new constitutive kinetochore protein CENP-X. Both CENP-Sand CENP-X-deficient chicken DT40 cells are viable but show abnormal mitotic behavior based on live cell analysis. Human HeLa cells depleted for CENP-X also showed mitotic errors. The kinetochore localization of CENP-S and -X is abolished in CENP-T-or CENP-K-deficient cells, but reciprocal experiments using CENP-S-deficient cells did not reveal defects in the localization of CCAN components. However, CENP-S- and CENP-X-deficient cells show a significant reduction in the size of the kine-to-chore outer plate. In addition, we found that intrakinetochore distance was increased in CENP-S- and CENP-X-deficient cells. These results suggest that the CENP-S complex is essential for the stable assembly of the outer kinetochore.

引用

页码：173 / 182

页数：10

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