Xanthan gum protects rabbit articular chondrocytes against sodium nitroprusside-induced apoptosis in vitro

被引：32

作者：

Chen, Qixin ^{[1
]}

Mei, Xifan ^{[2
]}

Han, Guanying ^{[2
,3
,4
]}

Ling, Peixue ^{[3
]}

Guo, Bin ^{[2
]}

Guo, Yuewei ^{[4
]}

Shao, Huarong ^{[3
]}

Wang, Guan ^{[1
]}

Cui, Zan ^{[2
]}

Bai, Yuxin ^{[1
]}

Xu, Fang ^{[1
]}

机构：

[1] Liaoning Med Univ, Sch Pharmaceut Sci, Jinzhou 121001, Peoples R China

[2] Liaoning Med Univ, Affiliated Hosp 1, Jinzhou 121001, Peoples R China

[3] Inst Biopharmaceut Shandong Prov, Postdoctoral Sci Res Workstat, Jinan 250101, Peoples R China

[4] Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2015年 / 131卷

基金：

中国国家自然科学基金;

关键词：

Xanthan gum; Sodium nitroprusside; Rabbit chondrocytes; Apoptosis; NITRIC-OXIDE; MITOCHONDRIAL DYSFUNCTION; CARTILAGE DEGRADATION; OSTEOARTHRITIS; INJECTION; RECOVERY; THERAPY;

D O I：

10.1016/j.carbpol.2015.06.004

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

We have previously reported that intra-articular injection of xanthan gum (XG) could significantly ameliorate the degree of joint cartilage degradation and pain in experimental osteoarthritis (OA) model in vivo. In this present study, we evaluated the protective effect of XG against Sodium nitroprusside (SNP)induced rabbit articular chondrocytes apoptosis in vitro. Rabbit articular chondrocytes were incubated with various concentrations of XG for 24 h prior to 0.5 mmol/L SNP co-treatment for 24 h. The proliferation of chondrocytes was analyzed using MU assay. The chondrocytes early apoptosis rates were evaluated using Annexin V-FITC/PI flow cytometry. The morphology of apoptosis chondrocytes were observed by scanning electron microscopy (SEM). The loss/disruption of mitochondrial membrane potential was detected using rhodamin 123 by confocal microscope. The concentration of prostaglandin E-2 (PGE(2)) in cell culture supernatants was evaluated using ELISA assay. The results showed that XG could significantly reverse SNP-reduced cell proliferation and inhibited cell early apoptosis rate in a dose-dependent manner. XG alleviated loss/disruption of mitochondrial membrane potential and decreased the PGE2 level of chondrocytes cell culture supernatants in SNP-induced chondrocytes. These results of the present research strongly suggest that XG can protect rabbit articular chondrocytes against SNP-induced apoptosis in vitro. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：363 / 369

页数：7

共 37 条

[1]

Prostaglandin E2 receptors EP1 and EP4 are up-regulated in rabbit chondrocytes by IL-1β, but not by TNFα [J].