Patterning of 293T fibroblasts on a mica surface

被引：6

作者：

Hou, Sen ^{[1
]}

Li, Xin-Xin ^{[1
]}

Li, Xiao-Yu ^{[1
]}

Feng, Xi-Zeng ^{[1
]}

Guan, Li ^{[2
]}

Yang, Yan-Lian ^{[2
]}

Wang, Chen ^{[2
]}

机构：

[1] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China

[2] Natl Ctr Nanosci & Technol, Beijing 100080, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2009年 / 394卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Cell adhesion; Microcontact printing; Collagen; Gene transfection; Mica; ATOMIC-FORCE MICROSCOPY; SOFT LITHOGRAPHY; CELL-CULTURE; PROTEINS; SYSTEMS;

D O I：

10.1007/s00216-009-2892-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Controllable cell growth on the defined areas of surfaces is important for potential applications in biosensor fabrication and tissue engineering. In this study, controllable cell growth was achieved by culturing 293 T fibroblast cells on a mica surface which had been patterned with collagen strips by a microcontact printing (mu CP) technique. The collagen area was designed to support cell adhesion and the native mica surface was designed to repel cell adhesion. Consequently, the resulting cell patterns should follow the micro-patterns of the collagen. X-ray photoelectron spectroscopy (XPS), water contact angle (WCA) measurement, atomic-force microscope (AFM) observation, and force-curve measurement were used to monitor property changes before and after the collagen adsorption process. Further data showed that the patterned cells were of good viability and able to perform a gene-transfection experiment in vitro. This technique should be of potential applications in the fields of biosensor fabrication and tissue engineering.

引用

页码：2111 / 2117

页数：7

共 27 条

[1] Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine [J].

Branch, DW ;

Wheeler, BC ;

Brewer, GJ ;

Leckband, DE .

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2000, 47 (03) :290-300

[2] Geometric control of cell life and death [J].

Chen, CS ;

Mrksich, M ;

Huang, S ;

Whitesides, GM ;

Ingber, DE .

SCIENCE, 1997, 276 (5317) :1425-1428

[3] Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems [J].

Chiu, DT ;

Jeon, NL ;

Huang, S ;

Kane, RS ;

Wargo, CJ ;

Choi, IS ;

Ingber, DE ;

Whitesides, GM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) :2408-2413

[4] Micro-patterning of animal cells on PDMS substrates in the presence of serum without use of adhesion inhibitors [J].

De Silva, MN ;

Desai, R ;

Odde, DJ .

BIOMEDICAL MICRODEVICES, 2004, 6 (03) :219-222

[5] A NEURON-SILICON JUNCTION - A RETZIUS CELL OF THE LEECH ON AN INSULATED-GATE FIELD-EFFECT TRANSISTOR [J].

FROMHERZ, P ;

OFFENHAUSSER, A ;

VETTER, T ;

WEIS, J .

SCIENCE, 1991, 252 (5010) :1290-1293

[6]

Ghosh P, 1999, ANGEW CHEM INT EDIT, V38, P1592, DOI 10.1002/(SICI)1521-3773(19990601)38:11<1592::AID-ANIE1592>3.0.CO

[7]

2-I

[8] Poly(ethylenimine) and its role in gene delivery [J].

Godbey, WT ;

Wu, KK ;

Mikos, AG .

JOURNAL OF CONTROLLED RELEASE, 1999, 60 (2-3) :149-160

[9] THE USE OF NEURONAL NETWORKS ON MULTIELECTRODE ARRAYS AS BIOSENSORS [J].

GROSS, GW ;

RHOADES, BK ;

AZZAZY, HME ;

WU, MC .

BIOSENSORS & BIOELECTRONICS, 1995, 10 (6-7) :553-567

[10] Kinetics of bone cell organization and mineralization on materials with patterned surface chemistry [J].

Healy, KE ;

Thomas, CH ;

Rezania, A ;

Kim, JE ;

McKeown, PJ ;

Lom, B ;

Hockberger, PE .

BIOMATERIALS, 1996, 17 (02) :195-208

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