Chimeric adenovirus vector encoding reovirus attachment protein σ1 targets cells expressing junctional adhesion molecule 1

被引:63
作者
Mercier, GT
Campbell, JA
Chappell, JD
Stehle, T
Dermody, TS [1 ]
Barry, MA
机构
[1] Rice Univ, Dept Bioengn, Houston, TX 77005 USA
[2] Vanderbilt Univ, Sch Med, Dept Microbiol & Immunol, Nashville, TN 37232 USA
[3] Vanderbilt Univ, Sch Med, Dept Pathol, Nashville, TN 37232 USA
[4] Vanderbilt Univ, Sch Med, Dept Pediat, Nashville, TN 37232 USA
[5] Vanderbilt Univ, Sch Med, Elizabeth B Lamb Ctr Pediat Res, Nashville, TN 37232 USA
[6] Massachusetts Gen Hosp, Lab Dev Immunol, Boston, MA 02114 USA
[7] Harvard Univ, Sch Med, Boston, MA 02114 USA
[8] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA
[9] Baylor Coll Med, Dept Immunol, Houston, TX 77030 USA
[10] Baylor Coll Med, Ctr Cell & Gene Therapy, Houston, TX 77030 USA
关键词
D O I
10.1073/pnas.0400542101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The utility of adenovirus (Ad) vectors for gene transduction can be limited by receptor specificity. We developed a gene-delivery vehicle in which the potent Ad5 vector was genetically reengineered to display the mucosal-targeting sigma1 protein of reovirus type 3 Dearing (T3D). A sigma1 construct containing all but a small virion-anchoring domain was fused to the N-terminal 44 aa of Ad5 fiber. This chimeric attachment protein Fibtail-T3Dsigma1 forms trimers and assembles onto Ad virions. Fibtail-T3Dsigma1 was recombined into the Ad5 genome, replacing sequences encoding wild-type fiber. The resulting vector, Ad5-T3Dsigma1, expresses Fibtail-T3Dsigma1 and infects Chinese hamster ovary cells transfected with human or mouse homologs of the reovirus receptor, junctional adhesion molecule 1 (JAM1), but not the coxsackievirus and Ad receptor. Treatment of Caco-2 intestinal epithelial cells with either JAM1-specific antibody or neuraminidase reduced transduction by Ad5T3Dsigma1, and their combined effect decreased transduction by 95%. Ad5-T3Dsigma1 transduces primary cultures of human dendritic cells substantially more efficiently than does Ad5, and this transduction depends on expression of JAM1. These data provide strong evidence that Ad5-T3Dsigma1 can be redirected to cells expressing JAM1 and sialic acid for application as a vaccine vector.
引用
收藏
页码:6188 / 6193
页数:6
相关论文
共 51 条
[31]  
Liu Y, 2000, J CELL SCI, V113, P2363
[32]   Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration [J].
Martìn-Padura, I ;
Lostaglio, S ;
Schneemann, M ;
Williams, L ;
Romano, M ;
Fruscella, P ;
Panzeri, C ;
Stoppacciaro, A ;
Ruco, L ;
Villa, A ;
Simmons, D ;
Dejana, E .
JOURNAL OF CELL BIOLOGY, 1998, 142 (01) :117-127
[33]  
Nibert M.L., 2001, FIELDS VIROLOGY, P1679
[34]  
Ozaki H, 1999, J IMMUNOL, V163, P553
[35]   Metabolically biotinylated adenovirus for cell targeting, ligand screening, and vector purification [J].
Parrott, MB ;
Adams, KE ;
Mercier, GT ;
Mok, H ;
Campos, SK ;
Barry, MA .
MOLECULAR THERAPY, 2003, 8 (04) :688-700
[36]   What makes the bacteriophage λ Red system useful for genetic engineering:: molecular mechanism and biological function [J].
Poteete, AR .
FEMS MICROBIOLOGY LETTERS, 2001, 201 (01) :9-14
[37]   Dendritic cells shuttle microbes across gut epithelial monolayers [J].
Rescigno, M ;
Rotta, G ;
Valzasina, B ;
Ricciardi-Castagnoli, P .
IMMUNOBIOLOGY, 2001, 204 (05) :572-581
[38]   Dendritic cells express tight junction proteins and penetrate gut epithelial monolayers to sample bacteria [J].
Rescigno, M ;
Urbano, M ;
Valzasina, B ;
Francolini, M ;
Rotta, G ;
Bonasio, R ;
Granucci, F ;
Kraehenbuhl, JP ;
Ricciardi-Castagnoli, P .
NATURE IMMUNOLOGY, 2001, 2 (04) :361-367
[39]   The coxsackievirus-adenovirus receptor protein can function as a cellular attachment protein for adenovirus serotypes from subgroups A, C, D, E, and F [J].
Roelvink, PW ;
Lizonova, A ;
Lee, JGM ;
Li, Y ;
Bergelson, JM ;
Finberg, RW ;
Brough, DE ;
Kovesdi, I ;
Wickham, TJ .
JOURNAL OF VIROLOGY, 1998, 72 (10) :7909-7915
[40]   Recent advances in the development of HIV-1 vaccines using replication-incompetent adenovirus vectors [J].
Shiver, JW ;
Emini, EA .
ANNUAL REVIEW OF MEDICINE, 2004, 55 :355-372