Molecular cloning and biological activity of ecdysis-triggering hormones in Drosophila melanogaster

被引：93

作者：

Park, Y

Zitnan, D

Gill, SJ

Adams, ME

机构：

[1] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Dept Neurosci, Riverside, CA 92521 USA

[3] Slovak Acad Sci, Inst Zool, Bratislava 84206, Slovakia

来源：

FEBS LETTERS | 1999年 / 463卷 / 1-2期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

ecdysis; eclosion; peptide; hormone; ecdysis-triggering hormone; behavior; Drosophila;

D O I：

10.1016/S0014-5793(99)01622-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ecdysis-triggering hormones (ETH) initiate a defined behavioral sequence leading to shedding of the insect cuticle. We have identified eth, a gene encoding peptides with ETH-like structure and biological activity in Drosophila melanogaster. The open reading frame contains three putative peptides based on canonical endopeptidase cleavage and amidation sites, Two of the predicted peptides (DrmETH1 and DrmETH2) prepared by chemical synthesis induce premature eclosion upon injection into pharate adults, The promoter region of the gene contains a direct repeat ecdysteroid response element. identification of eth in Drosophila provides opportunities for genetic manipulation of endocrine and behavioral events underlying a stereotypic behavior. (C) 1999 Federation of European Biochemical Societies.

引用

页码：133 / 138

页数：6

共 30 条

[1] Identification of ecdysis-triggering hormone in the silkworm Bombyx mori [J].