Reaction kinetics in intracellular environments with macromolecular crowding: simulations and rate laws

被引：274

作者：

Schnell, S

Turner, TE

机构：

[1] Univ Oxford, Inst Math, Ctr Math Biol, Oxford OX1 3LB, England

[2] Oxford Ctr Ind & Appl Math, Inst Math, Oxford OX1 3LB, England

[3] Univ Oxford Christ Church, Oxford OX1 1DP, England

来源：

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY | 2004年 / 85卷 / 2-3期

关键词：

macromolecular crowding; intracellular environment; reaction rate; fractal-like kinetics; enzymatic reactions;

D O I：

10.1016/j.pbiomolbio.2004.01.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We review recent evidence illustrating the fundamental difference between cytoplasmic and test tube biochemical kinetics and thermodynamics, and showing the breakdown of the law of mass action and power-law approximation in in vivo conditions. Simulations of biochemical reactions in non-homogeneous media show that as a result of anomalous diffusion and mixing of the biochemical species, reactions follow a fractal-like kinetics. Consequently, the conventional equations for biochemical pathways fail to describe the reactions in in vivo conditions. We present a modification to fractal-like kinetics following the Zipf-Mandelbrot distribution which will enable the modelling and analysis of biochemical reactions occurring in crowded intracellular environments. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：235 / 260

页数：26

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