The dynamic distribution of TrkB receptors before, during, and after synapse formation between cortical neurons

被引:69
作者
Gomes, Raquel A. [1 ]
Hampton, Cara [1 ]
El-Sabeawy, Faten [1 ]
Sabo, Shasta L. [1 ]
McAllister, A. Kimberley [1 ]
机构
[1] Univ Calif Davis, Ctr Neurosci, Davis, CA 95616 USA
关键词
synaptic vesicle transport; visual cortex; synaptogenesis; neurotrophin; BDNF; TrkB-EGFP; CULTURED HIPPOCAMPAL-NEURONS; PRESYNAPTIC ACTIVE ZONES; NEUROTROPHIC FACTOR; NMDA RECEPTORS; NERVOUS-SYSTEM; FULL-LENGTH; RETROGRADE TRANSPORT; DEPENDENT REGULATION; SYMPATHETIC NEURONS; INHIBITORY SYNAPSES;
D O I
10.1523/JNEUROSCI.2364-06.2006
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Although brain-derived neurotrophic factor (BDNF) potently regulates neuronal connectivity in the developing CNS, the mechanism by which BDNF influences the formation and/or maintenance of glutamatergic synapses remains unknown. Details about the subcellular localization of the BDNF receptor, TrkB, relative to synaptic and nonsynaptic proteins on excitatory neurons should provide insight into how BDNF might exert its effects during synapse formation. Here, we investigated the subcellular localization of tyrosine kinase receptor B (TrkB) relative to synaptic vesicle-associated proteins and NMDA receptors using immunocytochemistry, confocal microscopy, and time-lapse imaging in dissociated cultures of cortical neurons before, during, and after the peak of synapse formation. We find that TrkB is present in puncta on the surface and intracellularly in both dendrites and axons throughout development. Before synapse formation, some TrkB puncta in dendrites colocalize with NMDA receptors, and almost all TrkB puncta in axons colocalize with synaptic vesicle proteins. Clusters of TrkB fused to the enhanced green fluorescent protein (TrkB-EGFP) are highly mobile in both axons and dendrites. In axons, TrkB-EGFP dynamics are almost identical to vesicle-associated protein (VAMP2-EGFP), and these proteins are often transported together. Finally, surface TrkB is found in structures that actively participate in synapse formation: axonal growth cones and dendritic filopodia. Over time, surface TrkB becomes enriched at glutamatergic synapses, which contain both catalytic and truncated TrkB. These results suggest that TrkB is in the right place at the right time to play a direct role in the formation of glutamatergic synapses between cortical neurons.
引用
收藏
页码:11487 / 11500
页数:14
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