Structural and antigenic types of cell wall polysaccharides from viridans group streptococci with receptors for oral actinomyces and streptococcal lectins

被引:66
作者
Cisar, JO [1 ]
Sandberg, AL [1 ]
Reddy, GP [1 ]
Abeygunawardana, C [1 ]
Bush, CA [1 ]
机构
[1] UNIV MARYLAND BALTIMORE CTY, DEPT CHEM & BIOCHEM, BALTIMORE, MD 21228 USA
关键词
D O I
10.1128/IAI.65.12.5035-5041.1997
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Lectin-mediated interactions between oral viridans group streptococci and actinomyces may play an important role in microbial colonization of the tooth surface. The presence of two host-like motifs, either GalNAc beta 1-->3Gal (Gn) or Gal beta 1-->3GalNAc (G), in the cell wall polysaccharides of five streptococcal strains accounts for the lactose-sensitive coaggregations of these bacteria with Actinomyces naeslundii. Three streptococcal strains which have Gn-containing polysaccharides also participate in GalNAc-sensitive coaggregations with strains of Streptococcus gordonii and S. sanguis, Each Gn-or G-containing polysaccharide is composed of a distinct phosphodiester-linked hexa-or heptasaccharide repeating unit. The occurrence of these polysaccharides on 19 additional viridans group streptococcal strains that participate in lactose-sensitive coaggregations with actinomyces was examined, Negatively charged polysaccharides that reacted with Bauhinia purpurea agglutinin, a Gal and GalNAc binding plant lectin, were isolated from 17 strains by anion exchange column chromatography of mutanolysin-cell wall digests. Results from nuclear magnetic resonance and immunodiffusion identified each of 16 polysaccharides as a known Gn-or G-containing structural type and one polysaccharide as a new but closely related Gn-containing type. Unlike the reactions of lectins, the cross-reactions of most rabbit antisera with these polysaccharides were correlated with structural features other than the host-like motifs. Gn-containing polysaccharides occurred primarily on the strains of S. sanguis and S. oralis while G-containing polysaccharides were more common among the strains of S. gordonii and S. mitis examined. The findings strongly support the hypothesis that lectin-mediated recognition of these streptococci by other oral bacteria depends on a family of antigenically diverse Gn-and G-containing cell wall polysaccharides, the occurrence of which may differ between streptococcal species.
引用
收藏
页码:5035 / 5041
页数:7
相关论文
共 45 条
[41]   POLYSIALYLATION - FROM BACTERIA TO BRAINS [J].
TROY, FA .
GLYCOBIOLOGY, 1992, 2 (01) :5-23
[42]   Molecular modeling of the flexible cell wall polysaccharide of Streptococcus mitis J22 on the basis of heteronuclear NMR coupling constants [J].
Xu, QW ;
Bush, CA .
BIOCHEMISTRY, 1996, 35 (46) :14521-14529
[43]   Variation of gonococcal lipooligosaccharide structure is due to alterations in poly-G tracts in lgt genes encoding glycosyl transferases [J].
Yang, QL ;
Gotschlich, EC .
JOURNAL OF EXPERIMENTAL MEDICINE, 1996, 183 (01) :323-327
[44]  
YEUNG M, UNPUB
[45]  
YOKOGAWA K, 1974, ANTIMICROB AGENTS CH, V6, P156, DOI 10.1128/AAC.6.2.156