Myometrial maxi-K channel β1 subunit modulation during pregnancy and after 17β-estradiol stimulation

被引：24

作者：

Benkusky, NA ^{[1
]}

Korovkina, VP ^{[1
]}

Brainard, AM ^{[1
]}

England, SK ^{[1
]}

机构：

[1] Univ Iowa, Dept Physiol & Biophys, Iowa City, IA 52242 USA

来源：

FEBS LETTERS | 2002年 / 524卷 / 1-3期

关键词：

mouse; uterus; maxi-K; beta subunit; gestation; estrogen; progesterone;

D O I：

10.1016/S0014-5793(02)03011-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Myometrial maxi-K channels are modulated by beta subunits. We aimed to determine whether beta subunits are modulated to affect uterine excitability during gestation. RNase protection analyses revealed that mouse beta1 subunit transcripts are regulated during gestation with peak expression at day 14 of pregnancy. Immunohistochemical analysis indicates an increase of this subunit during gestation. Upregulation of the beta1 transcript occurs with 4-day exposure to 17beta-estradiol but not progesterone, and acute estradiol exposure has no effect on beta1 transcript expression. These findings verify that beta1 subunit transcript is regulated in mouse myometrium during gestation and estrogens may contribute to this increase. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：97 / 102

页数：6

共 18 条

[1] CALCIUM-ACTIVATED K+ CHANNELS AS MODULATORS OF HUMAN MYOMETRIAL CONTRACTILE ACTIVITY [J].

ANWER, K ;

OBERTI, C ;

PEREZ, GJ ;

PEREZREYES, N ;

MCDOUGALL, JK ;

MONGA, M ;

SANBORN, BM ;

STEFANI, E ;

TORO, L .

AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (04) :C976-C985

[2] GESTATIONAL PATTERN OF ESTRADIOL, TESTOSTERONE AND PROGESTERONE SECRETION IN SELECTED STRAINS OF MICE [J].

BARKLEY, MS ;

GESCHWIND, II ;

BRADFORD, GE .

BIOLOGY OF REPRODUCTION, 1979, 20 (04) :733-738

[3]

Benkusky NA, 2000, J BIOL CHEM, V275, P27712

[4] Differential expression of the alpha and beta subunits of the large-conductance calcium-activated potassium channel: Implication for channel diversity [J].

Chang, CP ;

Dworetzky, SI ;

Wang, JC ;

Goldstein, ME .

MOLECULAR BRAIN RESEARCH, 1997, 45 (01) :33-40

[5] Regulation of progesterone receptors and decidualization in uterine stroma of the estrogen receptor-α knockout mouse [J].

Kurita, T ;

Lee, KJ ;

Saunders, PTK ;

Cooke, PS ;

Taylor, JA ;

Lubahn, DB ;

Zhao, C ;

Mäkelä, S ;

Gustafsson, JÅ ;

Dahiya, R ;

Cunha, GR .

BIOLOGY OF REPRODUCTION, 2001, 64 (01) :272-283

[6] FUNCTIONAL-ROLE OF THE BETA-SUBUNIT OF HIGH-CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNELS [J].

MCMANUS, OB ;

HELMS, LMH ;

PALLANCK, L ;

GANETZKY, B ;

SWANSON, R ;

LEONARD, RJ .

NEURON, 1995, 14 (03) :645-650

[7] Histidine decarboxylase gene in the mouse uterus is regulated by progesterone and correlates with uterine differentiation for blastocyst implantation [J].

Paria, BC ;

Das, N ;

Das, SK ;

Zhao, X ;

Dileepan, KN ;

Dey, SK .

ENDOCRINOLOGY, 1998, 139 (09) :3958-3966

[8] CIRCULATING PLASMA-LEVELS OF PREGNENOLONE, PROGESTERONE, ESTROGEN, LUTEINIZING-HORMONE, AND FOLLICLE-STIMULATING HORMONE IN YOUNG AND AGED C57BL-6 MICE DURING VARIOUS STAGES OF PREGNANCY [J].

PARKENING, TA ;

LAU, IF ;

SAKSENA, SK ;

CHANG, MC .

JOURNALS OF GERONTOLOGY, 1978, 33 (02) :191-196

[9] PROGESTERONE-LIKE EFFECTS OF ESTRADIOL ON REPRODUCTIVE-BEHAVIOR AND HYPOTHALAMIC PROGESTIN RECEPTORS IN THE FEMALE RAT [J].

PARSONS, B ;

RAINBOW, TC ;

SNYDER, L ;

MCEWEN, BS .

NEUROENDOCRINOLOGY, 1984, 39 (01) :25-30

[10] Does estradiol mediate leptin's effects on adiposity and body weight? [J].

Pelleymounter, MA ;

Baker, MB ;

McCaleb, M .

AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1999, 276 (05) :E955-E963

← 1 2 →