The specific localization of seminolipid molecular species on mouse testis during testicular maturation revealed by imaging mass spectrometry

被引：57

作者：

Goto-Inoue, Naoko ^{[1
]}

Hayasaka, Takahiro ^{[1
]}

Zaima, Nobuhiro ^{[1
]}

Setou, Mitsutoshi ^{[1
]}

机构：

[1] Hamamatsu Univ Sch Med, Dept Mol Anat, Higashi Ku, Hamamatsu, Shizuoka 4313192, Japan

来源：

GLYCOBIOLOGY | 2009年 / 19卷 / 09期

基金：

日本科学技术振兴机构;

关键词：

imaging; MALDI; mass spectrometry; seminolipid; testis; CHAIN FATTY-ACIDS; DEFICIENT; CELLS; RAFTS; LONG;

D O I：

10.1093/glycob/cwp089

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

More than 90% of the glycolipid in mammalian testis consists of a unique sulfated glyceroglycolipid called seminolipid. The galactosylation of the molecule is catalyzed by UDP-galactose:ceramide galactosyltransferase (CGT). Disruption of the CGT gene in mice results in male infertility due to the arrest of spermatogenesis, indicating that seminolipid plays an important role in reproductive function. Seminolipid molecules can be assigned to different molecular species based on the fatty acid composition. In this report, we investigated the localizations of the molecular species of seminolipid by imaging mass spectrometry and demonstrated that major molecule (C16:0-alkyl-C16:0-acyl) was expressed throughout the tubules: some (C16:0-alkyl-C14:0-acyl and C14:0-alkyl-C16:0-acyl) were predominantly expressed in spermatocytes and the other (C17:0-alkyl-C16:0-acyl) was specifically expressed in spermatids and spermatozoa. This is the first report to show the cell-specific localization of each molecular species of seminolipid during testicular maturation.

引用

页码：950 / 957

页数：8

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