Membrane cofactor protein (CD46) is a basolateral protein that is not endocytosed - Importance of the tetrapeptide FTSL at the carboxyl terminus

被引:45
作者
Maisner, A
Zimmer, G
Liszewski, MK
Lublin, DM
Atkinson, JP
Herrler, G
机构
[1] UNIV MARBURG,INST VIROL,D-35037 MARBURG,GERMANY
[2] WASHINGTON UNIV,SCH MED,DEPT PATHOL & MED,DIV LAB MED,ST LOUIS,MO 63110
[3] WASHINGTON UNIV,SCH MED,DEPT INTERNAL MED,DIV RHEUMATOL,ST LOUIS,MO 63110
关键词
D O I
10.1074/jbc.272.33.20793
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Membrane cofactor protein (MCP) is a widely distributed complement regulatory protein that is expressed on the basolateral surface of polarized epithelial cells. The basolateral targeting of the BC1 isoform of MCP was analyzed by generating deletion mutants and point mutants within the cytoplasmic tail of 16 amino acids, A sequence of four amino acids, FTSL, was found to be indispensable for the basolateral transport of MCP. This tetrapeptide has two unique features compared with the targeting motifs of other basolateral proteins: (i) it contains a phenylalanine rather than a tyrosine at position 1; (ii) it is located at the very COOH-terminal end, Replacement of the phenylalanine or the leucine by an alanine resulted in a nonpolarized delivery to the cell surface, On the other hand, substitution of a tyrosine for the phenylalanine did not affect the basolateral transport of MCP, The latter mutant, however, was efficiently internalized, whereas the wild type protein was not subject to endocytosis. Our results indicate that the targeting signal YXX-large aliphatic that is involved in various sorting events has been modulated in MCP in such a way that it allows basolateral transport but not endocytosis.
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页码:20793 / 20799
页数:7
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