Purification and characterization of an inducible p-coumaric acid decarboxylase from Lactobacillus plantarum

被引：97

作者：

Cavin, JF ^{[1
]}

Barthelmebs, L ^{[1
]}

Guzzo, J ^{[1
]}

VanBeeumen, J ^{[1
]}

Samyn, B ^{[1
]}

Travers, JF ^{[1
]}

Divies, C ^{[1
]}

机构：

[1] STATE UNIV GHENT,BIOCHEM LAB,B-9000 GHENT,BELGIUM

来源：

FEMS MICROBIOLOGY LETTERS | 1997年 / 147卷 / 02期

关键词：

Lactobacillus plantarum; decarboxylase; p-coumaric acid; ferulic acid; caffeic acid;

D O I：

10.1016/S0378-1097(97)00004-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lactobacillus plantarum cells displayed substrate-inducible decarboxylase activities on p-coumaric and ferulic acids of 0.6 and 0.01 mu mol min(-1) mg(-1), respectively. Activity in uninduced cells or corresponding cell extracts was undetectable (< 10(-4) mu mol min(-1) mg(-1)). Specificity of induction indicates that at least two phenolic acid decarboxylases are produced in this bacterium. SDS-PAGE of partially purified protein extract from p-coumaric acid-induced cells showed one band of 23.5 kDa that was absent in the extract from uninduced cells, The native molecular mass of 93 kDa indicates that the enzyme is a homotetramer. The 1276-fold purified enzyme had a K-m of 1.4 mM, a V-m of about 766 mu mol min(-1) mg(-1), and a K-cat of 10(3) s(-1) for p-coumaric and caffeic acids, but did not display any detectable activity on ferulic acid. Maximum activity was at 30 degrees C, at pH 5.5-6, Cofactors or metal ions were not required for activity.

引用

页码：291 / 295

页数：5

共 13 条

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