The Saccharomyces cerevisiae OXA1 gene is required for the correct assembly of cytochrome c oxidase and oligomycin-sensitive ATP synthase

被引:121
作者
Altamura, N
Capitanio, N
Bonnefoy, N
Papa, S
Dujardin, G
机构
[1] UNIV BARI,IST BIOCHIM MED & CHIM,BARI,ITALY
[2] UNIV PARIS 06,CTR GENET MOLEC,CNRS,F-91198 GIF SUR YVETTE,FRANCE
关键词
respiratory complex assembly; cytochrome c oxidase; ATP synthase; OXA1; Saccharomyces cerevisiae;
D O I
10.1016/0014-5793(96)00165-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a posttranslational step in cytochrome c oxidase biogenesis, probably at the level of assembly, Mutations in OXA1 lead to a complete respiratory deficiency, The protein Oxa1p is conserved through evolution and a human homolog has been isolated by functional complementation of a yeast oxa1(-) mutant, In order to further our understanding of the role of Oxa1p, we have constructed two yeast strains in which the OXA1 open reading frame was almost totally deleted. Cytochrome spectra and enzymatic activity measurements show the absence of heme aa(3) and of a cytochrome c oxido-reductase activity and dramatic decrease of the oligomycin sensitive ATPase activity, Analysis of the respiratory complexes in non-denaturing gels reveals that Oxa1p is necessary for the correct assembly of the cytochrome c oxidase and the ATP synthase complex.
引用
收藏
页码:111 / 115
页数:5
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