Production, purification, and properties of an extracellular laccase from Rigidoporus lignosus

被引：55

作者：

Cambria, MT ^{[1
]}

Cambria, A ^{[1
]}

Ragusa, S ^{[1
]}

Rizzarelli, E ^{[1
]}

机构：

[1] Univ Catania, Dept Chem Sci, I-95125 Catania, Italy

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2000年 / 18卷 / 02期

关键词：

D O I：

10.1006/prep.1999.1126

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Laccase from Rigidoporus lignosus, a white-rot basidiomycete, has been isolated from culture filtrates. The enzyme was purified to homogeneity and some of its structural and kinetic parameters have been determined. The effects of pH, temperature, and organic solvents on the activity and stability of the enzyme, under different conditions, were also assayed. The results we have obtained, including the rather broad substrate specificity of enzyme, combined with their relatively easy production and purification, suggest that laccase may be efficiently employed in a variety of biotechnology applications. (C) 2000 Academic Press.

引用

页码：141 / 147

页数：7

共 33 条

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