Super-Resolution Fluorescence Microscopy

被引：1199

作者：

Huang, Bo ^{[1
,2
]}

Bates, Mark ^{[3
]}

Zhuang, Xiaowei ^{[1
,2
,4
]}

机构：

[1] Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA

[2] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

[3] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[4] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

来源：

ANNUAL REVIEW OF BIOCHEMISTRY | 2009年 / 78卷

关键词：

diffraction limit; fluorescence imaging; fluorescent protein; live-cell imaging; photoswitchable dye; single molecule; 3-DIMENSIONAL SUPERRESOLUTION; SUBDIFFRACTION-RESOLUTION; LOCALIZATION MICROSCOPY; DIFFRACTION BARRIER; LIGHT-MICROSCOPY; STED MICROSCOPY; PROTEIN; NANOSCOPY; MOLECULES; BREAKING;

D O I：

10.1146/annurev.biochem.77.061906.092014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Achieving a spatial resolution that is not limited by the diffraction of light, recent developments of super-resolution fluorescence microscopy techniques allow the observation of many biological structures not resolvable in conventional fluorescence microscopy. New advances in these techniques now give them the ability to image three-dimensional (3D) structures, measure interactions by multicolor colocalization, and record dynamic processes in living cells at the nanometer scale. It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.

引用

页码：993 / 1016

页数：24

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