Adipose Tissue of Control and Ex-Obese Patients Exhibit Differences in Blood Vessel Content and Resident Mesenchymal Stem Cell Population

被引：48

作者：

Baptista, Leandra Santos ^{[1
]}

da Silva, Karina Ribeiro ^{[1
,2
]}

Gouveia da Pedrosa, Carolina Silva ^{[1
,2
]}

Claudio-da-Silva, Cesar ^{[3
]}

Ivar Carneiro, Joao Regis ^{[4
]}

Aniceto, Marcelo ^{[3
]}

de Mello-Coelho, Valeria ^{[2
]}

Takiya, Christina Maeda ^{[2
]}

Doria Rossi, Maria Isabel ^{[1
,2
]}

Borojevic, Radovan ^{[1
,2
]}

机构：

[1] Univ Fed Rio de Janeiro, Clementino Fraga Filho Univ Hosp, APABCAM, BR-21941913 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Biomed Sci, BR-21941902 Rio De Janeiro, Brazil

[3] Univ Fed Rio de Janeiro, Clementino Fraga Filho Univ Hosp, Dept Gen Surg, Plast Surg Serv, BR-21941913 Rio De Janeiro, Brazil

[4] Univ Fed Rio de Janeiro, Clementino Fraga Filho Univ Hosp, Nutrol Dept, BR-21941913 Rio De Janeiro, Brazil

来源：

OBESITY SURGERY | 2009年 / 19卷 / 09期

关键词：

Adipose tissue; Obesity; Mesenchymal stem cells; Blood vessels; Weight regain; INFLAMMATION; ANGIOGENESIS; BONE;

D O I：

10.1007/s11695-009-9899-2

中图分类号：

R61 [外科手术学];

学科分类号：

100210 [外科学];

摘要：

The normal function of white adipose tissue is disturbed in obesity. After weight loss that follows bariatric surgery, ex-obese patients undergo plastic surgery to remove residual tissues and it is not known whether their adipose tissue returns to its original state. The aim of this study was to compare the white adipose tissue composition of ex-obese with control patients with regard to blood vessels and resident mesenchymal stem cells (MSC). Quantification of blood vessels was performed on histological sections of adipose tissue stained with hematoxylin and eosin and for von Willebrand antigen. MSC were induced to the adipogenic and osteogenic lineages by specific inductive culture media. Expression of PPAR gamma 2 was analyzed by reverse transcription polymerase chain reaction. Ex-obese adipose tissue showed a higher number (p = 0.0286) of small (107.3 +/- 22.0) and large (22.5 +/- 6.4) blood vessels, when compared to control patients (42.0 +/- 24.4 and 7.2 +/- 2.2, respectively) and they also occupied a larger area (control versus ex-obese, p = 0.0286). Adipose tissue MSC from both groups of patients expressed PPAR gamma 2 and were equally able to differentiate to the osteogenic lineage, but ex-obese MSC showed a higher adipogenic potential when induced in vitro (p < 0.05). The higher number of adipose tissue blood vessels in ex-obese patients explains the excessive bleeding observed during their plastic surgery. The presence of more committed cells to the adipogenic lineage may favor the easy weight regain that occurs in ex-obese patients. These results show that, after extensive weight loss, adipose tissue cell composition was not totally restored.

引用

页码：1304 / 1312

页数：9

共 22 条

[1]

Baptista L. S., 2007, J STEM CELLS, P73

[2]

Bougoulia Maria, 2006, Hormones (Athens), V5, P192

[3]

Angiogenesis in health and disease [J].