Ca2+-independent phospholipase A2 enhances store-operated Ca2+ entry in dystrophic skeletal muscle fibers

被引:103
作者
Boittin, Francois-Xavier [1 ]
Petermann, Olivier [1 ]
Hirn, Carole [1 ]
Mittaud, Peggy [1 ]
Dorchies, Olivier M. [1 ]
Roulet, Emmanuelle [1 ]
Ruegg, Urs T. [1 ]
机构
[1] Univ Geneva, Univ Lausanne, Geneva Lausanne Sch Pharmeceut Sci, Pharmacol Lab, CH-1211 Geneva 4, Switzerland
关键词
dystrophic skeletal muscle fibers; store-operated channel; Ca2+-independent phospholipase A(2);
D O I
10.1242/jcs.03184
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Duchenne muscular dystrophy is caused by deficiency of dystrophin and leads to progressive weakness. It has been proposed that the muscle degeneration occurring in this disease is caused by increased Ca2+ influx due to enhanced activity of cationic channels that are activated either by stretch of the plasma membrane (stretch-activated channels) or by Ca2+-store depletion (store-operated channels). Using both cytosolic Ca2+ measurements with Fura-2 and the manganese quench method, we show here that store-operated Ca2+ entry is greatly enhanced in dystrophic skeletal flexor digitorum brevis fibers isolated from mdx(5cv) mice, a mouse model of Duchenne muscular dystrophy. Moreover, we show for the first time that store-operated Ca2+ entry in these fibers is under the control of the Ca2+-independent phospholipase A(2) and that the exaggerated Ca2+ influx can be completely attenuated by inhibitors of this enzyme. Enhanced store-operated Ca2+ entry in dystrophic fibers is likely to be due to a near twofold overexpression of Ca2+-independent phospholipase A(2). The Ca2+-independent phospholipase A(2) pathway therefore appears as an attractive target to reduce excessive Ca2+ influx and subsequent degeneration occurring in dystrophic fibers.
引用
收藏
页码:3733 / 3742
页数:10
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