A pathogen-inducible endogenous siRNA in plant immunity

被引：341

作者：

Katiyar-Agarwal, Surekha

Morgan, Rebekah

Dahlbeck, Douglas

Borsani, Omar

Villegas, Andy, Jr.

Zhu, Jian-Kang

Staskawicz, Brian J. ^{[1
]}

Jin, Hailing

机构：

[1] Univ Calif Riverside, Dept Plant Pathol, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Dept Bot & Plant Sci, Ctr Plant Cell Biol, Riverside, CA 92521 USA

[3] Univ Calif Riverside, Inst Integrat Genome Biol, Riverside, CA 92521 USA

[4] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2006年 / 103卷 / 47期

关键词：

antibacterial defense; DCL1; RDR6; RPS2-specific;

D O I：

10.1073/pnas.0608258103

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

RNA interference, mediated by small interfering RNAs (siRNAs), is a conserved regulatory process that has evolved as an antiviral defense mechanism in plants and animals. it is not known whether host cells also use siRNAs as an antibacterial defense mechanism in eukaryotes. Here, we report the discovery of an endogenous siRNA, nat-siRNAATGB2, that is specifically induced by the bacterial pathogen Pseudomonas syringae carrying effector avrRpt2. We demonstrate that the biogenesis of this siRNA requires DCL1, HYL1, HEN1, RDR6, NRPD1A, and SGS3. Its induction also depends on the cognate host disease resistance gene RPS2 and the NDR1 gene that is required for RPS2-specified resistance. This siRNA contributes to RP52-mediated race-specific disease resistance by repressing PPRL, a putative negative regulator of the RPS2 resistance pathway.

引用

页码：18002 / 18007

页数：6

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