Two distinct actin networks drive the protrusion of migrating cells

被引：606

作者：

Ponti, A ^{[1
]}

Machacek, M ^{[1
]}

Gupton, SL ^{[1
]}

Waterman-Storer, CM ^{[1
]}

Danuser, G ^{[1
]}

机构：

[1] Scripps Res Inst TSRI, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

SCIENCE | 2004年 / 305卷 / 5691期

关键词：

D O I：

10.1126/science.1100533

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cell migration initiates by extension of the actin cytoskeleton at the leading edge. Computational analysis of fluorescent speckle microscopy movies of migrating epithelial cells revealed this process is mediated by two spatially colocalized but kinematically, kinetically, molecularly, and functionally distinct actin networks. A lamelhpodium network assembled at the leading edge but completely disassembled within 1 to 3 micrometers. It was weakly coupled to the rest of the cytoskeleton and promoted the random protrusion and retraction of the leading edge. Productive cell advance was a function of the second colocalized network, the lamella, where actomyosin contraction was integrated with substrate adhesion.

引用

页码：1782 / 1786

页数：5

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