Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A2 and Δ12-PGJ2

被引：41

作者：

Ahn, SG

Kim, HS

Jeong, SW

Kim, BE

Rhim, H

Shim, JY

Kim, JW

Lee, JH

Kim, IK

机构：

[1] Catholic Univ Korea, Coll Med, Dept Biochem, Socho Gu, Seoul 137701, South Korea

[2] Catholic Univ Korea, Coll Med, Res Inst Mol Genet, Socho Gu, Seoul 137701, South Korea

[3] Catholic Univ Korea, Coll Med, Dept Anesthesiol, Socho Gu, Seoul 137701, South Korea

来源：

EXPERIMENTAL AND MOLECULAR MEDICINE | 2002年 / 34卷 / 03期

关键词：

Sox-4; PGA(2); Delta(12)-PGJ(2); apoptosis;

D O I：

10.1038/emm.2002.34

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A2 and Delta(12)-PGJ(2) induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca2+ ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA(2) and (12)-PGJ(2) in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway. These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.

引用

页码：243 / 249

页数：7

共 29 条

[1] Identification of cDNAs for Sox-4, an HMG-Box protein, and a novel human homolog of yeast splicing factor SSF-1 differentially regulated during apoptosis induced by prostaglandin A2/Δ12-PGJ2 in Hep3B cells [J].