Multiplex single nucleotide polymorphisms genotyping using solid-phase single base extension on magnetic nanoparticles

被引：85

作者：

Liu, Hongna ^{[1
]}

Li, Song ^{[1
,2
]}

Liu, Lishang ^{[2
]}

Tian, Lan ^{[1
]}

He, Nongyue ^{[1
,2
]}

机构：

[1] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Peoples R China

[2] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412008, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 386卷 / 01期

基金：

湖南省自然科学基金; 美国国家科学基金会; 中国博士后科学基金;

关键词：

DNA;

D O I：

10.1016/j.ab.2008.11.035

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To fulfill the increasing need for large-scale genetic research, we have developed a new solid-phase single base extension (SBE) protocol on magnetic nanoparticles (MNPs) for multiplex SNP detection using adapter polymerase chain reaction (PCR) products as templates. Extension primers were covalently immobilized on the MNPs, and allele-specific extension took place along the stretch of target DNA for one-color ddNTP incorporation. The MNPs with fluorophores were spotted on a glass slide to fabricate a "bead array" to discriminate their genotypes. Eight SNP loci of three DNA samples were interrogated, and the experiment demonstrated that it is an efficient method for large-scale SNP genotyping. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：126 / 128

页数：3

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