Droplets for Ultrasmall-Volume Analysis

被引：159

作者：

Chiu, Daniel T. ^{[1
]}

Lorenz, Robert M. ^{[1
]}

Jeffries, Gavin D. M. ^{[1
]}

机构：

[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 13期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

IN-VITRO COMPARTMENTALIZATION; POLYMERASE-CHAIN-REACTION; MICROFLUIDIC DEVICE; AQUEOUS DROPLETS; PICOLITER DROPLETS; CAPILLARY-ELECTROPHORESIS; SUBCELLULAR ORGANELLES; CHEMICAL CONCENTRATION; VORTEX-TRAP; SINGLE-CELL;

D O I：

10.1021/ac900306q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

By using methods that permit the generation and manipulation of ultrasmall-volume droplets, researchers are pushing the boundaries of ultrasensitive chemical analyses. (To listen to a podcast about this feature, please go to the Analytical Chemistry Web site at pubs.acs.org/ancham.)

引用

收藏

页码：5111 / 5118

页数：8

相关论文

共 58 条

[1] Dielectrophoretic manipulation of drops for high-speed microfluidic sorting devices [J].

Ahn, K ;

Kerbage, C ;

Hunt, TP ;

Westervelt, RM ;

Link, DR ;

Weitz, DA .

APPLIED PHYSICS LETTERS, 2006, 88 (02) :1-3

[2] On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets [J].

Beer, N. Reginald ;

Wheeler, Elizabeth K. ;

Lee-Houghton, Lorenna ;

Watkins, Nicholas ;

Nasarabadi, Shanavaz ;

Hebert, Nicole ;

Leung, Patrick ;

Arnold, Don W. ;

Bailey, Christopher G. ;

Colston, Bill W. .

ANALYTICAL CHEMISTRY, 2008, 80 (06) :1854-1858

[3] On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets [J].

Beer, N. Reginald ;

Hindson, Benjamin J. ;

Wheeler, Elizabeth K. ;

Hall, Sara B. ;

Rose, Klint A. ;

Kennedy, Ian M. ;

Colston, Bill W. .

ANALYTICAL CHEMISTRY, 2007, 79 (22) :8471-8475

[4] Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery [J].

Bernath, K ;

Magdassi, S ;

Tawfik, DS .

JOURNAL OF MOLECULAR BIOLOGY, 2005, 345 (05) :1015-1026

[5] In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting [J].

Bernath, K ;

Hai, MT ;

Mastrobattista, E ;

Griffiths, AD ;

Magdassi, S ;

Tawfik, DS .

ANALYTICAL BIOCHEMISTRY, 2004, 325 (01) :151-157

[6] Spatially resolved detection of attomole quantities of organic molecules localized in picoliter vials using time-of-flight secondary ion mass spectrometry [J].

Braun, RM ;

Beyder, A ;

Xu, JY ;

Wood, MC ;

Ewing, AG ;

Winograd, N .

ANALYTICAL CHEMISTRY, 1999, 71 (16) :3318-3324

[7] The chemistrode: A droplet-based microfluidic device for stimulation and recording with high temporal, spatial, and chemical resolution [J].

Chen, Delai ;

Du, Wenbin ;

Liu, Ying ;

Liu, Weishan ;

Kuznetsov, Andrey ;

Mendez, Felipe E. ;

Philipson, Louis H. ;

Ismagilov, Rustem F. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (44) :16843-16848

[8] Micro- and nano-scale chemical analysis of individual sub-cellular compartments [J].

Chiu, DT .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2003, 22 (08) :528-536

[9] Chemical transformations in individual ultrasmall biomimetic containers [J].

Chiu, DT ;

Wilson, CF ;

Ryttsén, F ;

Strömberg, A ;

Farre, C ;

Karlsson, A ;

Nordholm, S ;

Gaggar, A ;

Modi, BP ;

Moscho, A ;

Garza-López, RA ;

Orwar, O ;

Zare, RN .

SCIENCE, 1999, 283 (5409) :1892-1895

[10] Microfluidic methods for generating continuous droplet streams [J].

Christopher, G. F. ;

Anna, S. L. .

JOURNAL OF PHYSICS D-APPLIED PHYSICS, 2007, 40 (19) :R319-R336

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