Molecular cytogenetic characterization of a complex rearrangement involving chromosomes 9 and 22 in a case of Ph-negative chronic myeloid leukemia

被引：12

作者：

Storlazzi, CT

Anelli, L

Surace, C

Lonoce, A

Zagaria, A

Nanni, M

Curzi, P

Rocchi, M ^{[1
]}

机构：

[1] Univ Bari, DAPEG, Sez Genet, Bari, Italy

[2] Univ Foggia, Dept Hematol, Foggia, Italy

[3] Univ Roma La Sapienza, Dept Cellular Biotechnol & Hematol, Rome, Italy

来源：

CANCER GENETICS AND CYTOGENETICS | 2002年 / 136卷 / 02期

关键词：

D O I：

10.1016/S0165-4608(02)00530-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The "golden path", produced by the Human Genome Project effort, is composed of a collection of overlapping and fully sequenced BAC/PAC clones covering almost completely the human genome. These clones can be advantageously exploited as fluorescence in situ hybridization (FISH) probes for the characterization of rearrangements frequently found in tumors. Breakpoint characterization can be further refined by generating additional smaller FISH probes through LONG-PCR amplification of specific DNA segments, 5-10 kb in size, using appropriate BAC/PAC probes as template. We report here an example of this approach that has been used to characterize a complex Ph-negative chronic myeloid leukemia (CML Ph-) case in which the BCR/ABL fusion gene was found located on chromosome 9. (C) 2002 Elsevier Science Inc. All rights reserved.

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页码：141 / 145

页数：5

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