Immunoblot evaluation of the species-specificity of Em18 and Em16 antigens for serodiagnosis of human alveolar echinococcosis

被引：22

作者：

Nirmalan, N

Craig, PS

机构：

[1] Department of Biological Sciences, University of Salford, Salford

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 1997年 / 91卷 / 04期

关键词：

echinococcosis; Echinococcus multilocularis; antigens; Em18; Em16; serodiagnosis;

D O I：

10.1016/S0035-9203(97)90293-5

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 [公共卫生与预防医学]; 120402 [社会医学与卫生事业管理];

摘要：

An immunoblot study to confirm the species-specificity of the diagnostic antigens Em18 and Em16 of Echinococcus multilocularis protoscolex extract showed that both antigens cross-reacted with sera from cystic echinococcosis (CE) patients. The 18 kDa component was detectable by 75% of the sera from active alveolar echinococcosis (AE) patients, while only 31% detected Em16. Western blot analysis also showed that AE sera recognized a band in the 18 kDa region of E. granulosus protoscolex extract, which was different from the 16/17 kDa subunit of antigen B. The results suggested that Em18 antigen is present in E. granulosus as well as E. multilocularis, and that some CE patients may have serum antibody against this antigen.

引用

页码：484 / 486

页数：3

共 15 条

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COPROANTIGEN DETECTION FOR IMMUNODIAGNOSIS OF ECHINOCOCCOSIS AND TAENIASIS IN DOGS AND HUMANS [J].