PL-S2, a homogeneous polysaccharide from Radix Puerariae lobatae, attenuates hyperlipidemia via farnesoid X receptor (FXR) pathway-modulated bile acid metabolism

被引：71

作者：

Rao, Yifei ^{[1
]}

Wen, Quan ^{[2
]}

Liu, Ronghua ^{[1
]}

He, Mingzhen ^{[1
]}

Jiang, Zhihui ^{[2
]}

Qian, Kai ^{[1
]}

Zhou, Chaoqun ^{[1
]}

Li, Junmao ^{[1
]}

Du, Hui ^{[1
]}

Ouyang, Hui ^{[1
]}

Feng, Yulin ^{[2
]}

Zhu, Weifeng ^{[3
]}

机构：

[1] Jiangxi Univ Tradit Chinese Med, 818 Yunwan Rd, Nanchang 330002, Jiangxi, Peoples R China

[2] State Key Lab Innovat Drug & Efficient Energy Sav, 56 Yangming Rd, Nanchang 330006, Jiangxi, Peoples R China

[3] Jiangxi Univ Tradit Chinese Med, Key Lab Modern Preparat Tradit Chinese Med, 818 Yunwan Rd, Nanchang 330002, Jiangxi, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 165卷

关键词：

Radix Puerariae lobatae; Polysaccharide; Food function; Hyperlipidemia; Bile acid metabolism; FXR signaling pathway; SULFATED POLYSACCHARIDE; PURIFICATION; ANTICOAGULANT; HOMEOSTASIS; COMPLEX; LIVER; RATS;

D O I：

10.1016/j.ijbiomac.2020.10.029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Polysaccharides are important active constituents of Radix Puerariae lobatae (RPL). In this study, a novel homogeneous polysaccharide from RPL was successfully obtained by HP-20 macroporous resin and purified by Sepharose G-100 column chromatography. Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of alpha-1,3-linked and alpha-1,4-linked glucose. The molecular weight of PL-S2 was 18.73 kDa. The hypolipidemic effect of PL-S2 on hyperlipidemic rats was evaluated in histopathology and metabolomics analyses. PL-S2 significantly reduced plasma lipid levels and inhibited bile acid metabolism. We also demonstrated that treatment with PL-S2 activated FXR, CYP7A1, BESP, and MRP2 in rat liver. Our findings first indicate that PL-S2 decreases plasma lipid levels in hyperlipidemic rats by activating the FXR signaling pathway and promoting bile acid excretion. Therefore, PL-S2 derived from RPL is implicated as a functional food factor with lipid-regulating activity, and highlighted as a potential food supplement for the treatment of hyperlipidemia. (C) 2020 Elsevier B.V. All rights reserved.

引用

页码：1694 / 1705

页数：12

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