Characterization of cholylglycine hydrolase from a bile-adapted strain of Xanthomonas maltophilia and its application for quantitative hydrolysis of conjugated bile salts

被引：37

作者：

Dean, M

Cervellati, C

Casanova, E

Squerzanti, M

Lanzara, V

Medici, A

de Laureto, PP

Bergamini, CM

机构：

[1] Univ Ferrara, Dept Biochem & Mol Biol, I-44100 Ferrara, Italy

[2] Univ Ferrara, Dept Chem, I-44100 Ferrara, Italy

[3] Univ Ferrara, Ctr Study Inflammatory Dis, ICSI, I-44100 Ferrara, Italy

[4] Univ Padua, Ctr Biotechnol, CRIBI, Padua, Italy

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 06期

关键词：

D O I：

10.1128/AEM.68.6.3126-3128.2002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Purified bile salt hydrolase from bile-adapted Xanthomonas maltophilia displays Michaelis-Menten kenetics on cholylglycine and cholyltaurine and hydrolyzes bile salts also in crude bovine bile. The protein is a dimer and is resistant to proteinases and to heating at 55 to 60degreesC for up to 60 min, in agreement with calorimetric data.

引用

页码：3126 / 3128

页数：3

共 24 条

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