Dual colour, microarray-based, analysis of 10 000 protease substrates

被引：42

作者：

Diaz-Mochon, Juan J. ^{[1
]}

Bialy, Laurent ^{[1
]}

Bradley, Mark ^{[1
]}

机构：

[1] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

来源：

CHEMICAL COMMUNICATIONS | 2006年 / 38期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1039/b609029j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A 10 000 member PNA-encoded library of FRET based peptides was synthesised for global analysis of protease cleavage specificity; analysis was achieved using a DNA microarray and consumed minimal quantities of enzyme (60 pmole) and library (3.5 nmole).

引用

页码：3984 / 3986

页数：3

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