Biological function of unannotated transcription during the early development of Drosophila melanogaster

被引:148
作者
Manak, J. Robert [1 ]
Dike, Sujit
Sementchenko, Victor
Kapranov, Philipp
Biemar, Frederic
Long, Jeff
Cheng, Jill
Bell, Ian
Ghosh, Srinka
Piccolboni, Antonio
Gingeras, Thomas R.
机构
[1] Affymetrix Inc, Santa Clara, CA 95051 USA
[2] Univ Calif Berkeley, Ctr Integrat Genom, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
关键词
D O I
10.1038/ng1875
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Many animal and plant genomes are transcribed much more extensively than current annotations predict. However, the biological function of these unannotated transcribed regions is largely unknown. Approximately 7% and 23% of the detected transcribed nucleotides during D. melanogaster embryogenesis map to unannotated intergenic and intronic regions, respectively. Based on computational analysis of coordinated transcription, we conservatively estimate that 29% of all unannotated transcribed sequences function as missed or alternative exons of well-characterized protein-coding genes. We estimate that 15.6% of intergenic transcribed regions function as missed or alternative transcription start sites (TSS) used by 11.4% of the expressed protein-coding genes. Identification of P element mutations within or near newly identified 5' exons provides a strategy for mapping previously uncharacterized mutations to their respective genes. Collectively, these data indicate that at least 85% of the fly genome is transcribed and processed into mature transcripts representing at least 30% of the fly genome.
引用
收藏
页码:1151 / 1158
页数:8
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