ATXR5 and ATXR6 are H3K27 monomethyltransferases required for chromatin structure and gene silencing

被引：227

作者：

Jacob, Yannick ^{[1
]}

Feng, Suhua ^{[2
,3
]}

LeBlanc, Chantal A. ^{[1
]}

Bernatavichute, Yana V. ^{[3
,4
]}

Stroud, Hume ^{[3
]}

Cokus, Shawn ^{[3
]}

Johnson, Lianna M. ^{[5
]}

Pellegrini, Matteo ^{[3
]}

Jacobsen, Steven E. ^{[2
,3
,4
]}

Michaels, Scott D. ^{[1
]}

机构：

[1] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA

[2] Univ Calif Los Angeles, Howard Hughes Med Inst, Los Angeles, CA 90024 USA

[3] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90024 USA

[4] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90024 USA

[5] Univ Calif Los Angeles, Life Sci Core Curriculum, Los Angeles, CA 90024 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2009年 / 16卷 / 07期

关键词：

HISTONE H3 METHYLATION; POLYCOMB-GROUP PROTEINS; SET-DOMAIN PROTEINS; DNA METHYLATION; EPIGENETIC INHERITANCE; LYSINE-9; METHYLATION; CPG METHYLATION; METHYLTRANSFERASE; MARKS; TRIMETHYLATION;

D O I：

10.1038/nsmb.1611

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Constitutive heterochromatin in Arabidopsis thaliana is marked by repressive chromatin modifications, including DNA methylation, histone H3 dimethylation at Lys9 (H3K9me2) and monomethylation at Lys27 (H3K27me1). The enzymes catalyzing DNA methylation and H3K9me2 have been identified; alterations in these proteins lead to reactivation of silenced heterochromatic elements. The enzymes responsible for heterochromatic H3K27me1, in contrast, remain unknown. Here we show that the divergent SET-domain proteins ARABIDOPSIS TRITHORAX-RELATED PROTEIN 5 (ATXR5) and ATXR6 have H3K27 monomethyltransferase activity, and atxr5 atxr6 double mutants have reduced H3K27me1 in vivo and show partial heterochromatin decondensation. Mutations in atxr5 and atxr6 also lead to transcriptional activation of repressed heterochromatic elements. Notably, H3K9me2 and DNA methylation are unaffected in double mutants. These results indicate that ATXR5 and ATXR6 form a new class of H3K27 methyltransferases and that H3K27me1 represents a previously uncharacterized pathway required for transcriptional repression in Arabidopsis.

引用

页码：763 / U96

页数：7

共 55 条

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